PRF Centrifuge System

What is the PRF Protocol?

The concept of PRF (Platelet Rich Fibrin) is based on the centrifugation of whole blood without anticoagulants nor bovine thrombin (nor any other gelling agent). At the end of the spin, a fibrin clot containing the majority of the platelets and white blood cells is obtained.  PRF acts as a scaffold for growth factors, which accelerate wound healing, including platelet derived growth factor (PDGF), transforming growth factor beta1 (TGF-β1), and vascular endothelial growth factor [1-5]. The expected objective of these growth factors is to accelerate the soft tissue and bone healing. Numerous international publications are available and demonstrate the effectiveness of PRF in many indications: oral surgery (implants, bone grafting, periodontal surgery, extractions, etc…), Orthoepedic, cosmetic and dermatological surgery.Today, PRF is recognized around the world as a proven technique for bone regeneration.

What is needed to create PRF ?

The technology required to produce PRF is simply an appropriate centrifuge and a set of instruments. The PRF protocol is very simple: A blood sample is taken without anticoagulant in 10-mL tubes which are immediately centrifuged at a specific RPM and time limit (the standard A-PRF ^{TM *} protocol, on the appropriate centrifuges with certain specifications, calls for 1300 rpm / 8mn, while the i-PRF ^{TM *} protocol is 700 rpm / 3mn for females and 700 rpm / 4mn for males).

According to a paper by DavidM.Dohan,DDS et al. (emphasis ours): "The absence of anticoagulant implies the activation in a few minutes of most platelets of the blood sample in contact with the tube walls and the release of the coagulation cascades. The success of this technique entirely depends on the speed of blood collection and transfer to the centrifuge. Indeed, without anticoagulant, the blood samples start to coagulate almost immediately upon contact with the tube glass, and it takes a minimum of a few minutes of centrifugation to concentrate fibrinogen in the middle and upper part of the tube. Quick handling is the only way to obtain a clinically usable PRF clot. If the duration required to collect blood and launch centrifugation is overly long, failure will occur: The fibrin will polymerize in a diffuse way in the tube and only a small blood clot without consistency will be obtained." ¹

Choukroun A - PRF ^{TM *} i-PRF ^{TM *} and Demonstration Videos

Red 10ml Glass TubesThe red glass tubes are used for making PRF clots/membrane. Box of 100 tubes.

White Plastic Tubes, 9ml The white tubes are used for making the liquid PRF. Box of 50 tubes

Blood Collectors

Venipuncture can be done by the Dental Surgeon (if he is trained) or by the nurse. Material: fast collector BC10. Boxes of 24 collectors.

Tourniquet

The tourniquet is used to perform a blood drawing.

Tube Holder

Is used to hold the tubes and let them rest after the blood drawing.

PRF Mini Tray

Used to prepare membranes, Separate the fibrin clot from the red blood cells - Cut the membranes - Fold the membranes - Make «punches»

PRF Bowl

Is used to mix the membranes cut into small pieces with biomaterials. It can also be used for the cutting: Put the membrane into the bowl and cut it with PRF scissors.

PRF Scissors

Allows to separate easily the fibrin clot from the red cells and to cut membranes into small pieces.

PRF Forceps

Is used to remove and manipulate membranes.

PRF Forceps “Giraffe”

The forceps giraffe is intended to tunneling the PRF membranes for surgical soft tissues procedures: introduce the forceps in the tunnel, open the forceps, block the PRF membranes and pull to place the membranes in the correct position.

PRF Double Spoon

Allows collecting and handling the bone filling materials in the recipient sites (alveoli, sinus, and goshawks implants).

PRF Compactor Big

Allows compacting and tamping the bone filling materials in the recipient sites (alveoli, sinus, and goshawks implants).

PRF Compactor Small

Allows compacting and tamping the bone filling materials in the recipient sites (alveoli, sinus, and goshawks implants).

PRF Pad

Is used to position the PRF membranes easily, anywhere,using the pad as a cake server. The pointed end of the pad can be introduced in the sinus.

DM0412 Clinical Centrifuge Features:

• Easy programming to achieve high accuracy of speed control CPU controls all operating parameters including speed and time
• Rotor speed can be set and displayed by RPM or G-force Operation can be timed from 30 seconds to 99 minutes or continuous running
• Timer commences once the set speed is reached, so the separation time is more accurate
• Gentle braking at low speeds with efficient separation
• Parameters can be modified after the set speed is reached
• Quick spins are possible by pressing and holding PULSE key Lid with electric lock and automatic release function saves processing time and prevents samples overheating
• Easy operation with intuitive menu and easy-to-read backlit LCD processing display and sound alert
• High strength plastic rotor and excellent balance technology for quiet and stable operation
• Maintenance-free brushless DC motors to drive quickly and effortlessly accelerates the rotor to set speed
• Automatic self-diagnosis on startup showing accumulative running time and last running parameters
• Comes with adapters and plugs for smaller tubes
• cTUVus approved for UL and CSA 2 YEAR WARRANTY

Specifications: 

(compare these specifications to other private-labeled centrifuges on the market and you will see that they are exactly same.).

^* A-PRF ^TM and i-PRF ^TM are registered trademarks of Process for PRF.

^** Choukroun et al. described a technique for generating PRF by centrifuging autologous blood without an anticoagulant or other chemical agents in glass tubes ^1,2,3. The yellowish fibrin clot forms in the middle of the tube a few minutes after centrifugation.

References for the PRF Centrifuge System

1. Dohan Ehrenfest DM, Choukroun J, Diss A, Dohan SL, Dohan AJJ, Mouhyi J, et al. Platelet-rich fibrin (PRF): A second- generation platelet concentrate. Part I: Technological concepts and evolution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;101:37-44.

2. Dohan Ehrenfest DM, Choukroun J, Diss A, Dohan AJJ, Mouhyi J, Gogly B. Platelet- rich fibrin (PRF): A second-generation platelet concentrate. Part II: Platelet- related biologic features. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;101:45-50.

3. Choukroun J, Diss A, Simonpieri A. Platelet-rich fibrin (PRF): A second- generation platelet concentrate. Part V: Histologic evaluations of PRF effects on bone allograft maturation in sinus lift. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;101:299-303.

4. Dohan Ehrenfest DM, Peppo de GM, Doglioli P, Sammartino G. Slow release of growth factors and thrombospondin-1 in Choukroun’s platelet-rich fibrin (PRF): A gold standard to achieve for all surgical platelet concentrates technologies. Growth Factors. 2009;27:63-9. 4.

5. Su CY, Kuo YP, Tseng YH, CH Su, Burnouf T. In vitro release of growth factors from platelet-rich fibrin (PRF): A proposal to optimize the clinical applications of PRF. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009; 108:56-61.